• Nutrient agar is used to culture a wide range of bacteria, molds and yeasts. The 350ml bottle of agar can fill 14 to 15 90x15mm petri dishes. Useful shelf life is approximately one year.

Nutrient Agar, Ready-To-Pour Kit

  • Z0050
Price: $29.95
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The kit includes...

  • One 350ml bottle of ready-to-pour nutrient agar.
  • 20 sterile, 90x15mm plastic Petri dishes.
  • 20 individually wrapped, sterile swabs.
  • Directions for preparation and use.

Instructions for Using Ready-To-Pour Nutrient Agar - 350ml Bottle

Items needed...

  • Bottle of Ready-To-Pour Nutrient Agar.
  • Sterile swabs - At least one per dish.
  • Sterile Petri Dishes.
  • Masking Tape.

The 350ml bottle can serve to fill 12 to 15 Petri dishes with approximately 25ml of agar to depth of 6-7mm.


  • Leave cap on bottle and loosen prior to placing in a microwave. To accommodate variations in the latter, melt bottle contents in one minute intervals which may be repeated until contents liquify. Allow 2-3 minutes for melting between microwave heating. Use hot-pad gloves to handle bottle between these insertions.
  • If cap is not loosened, air won’t vent and bottle will crack. A double-boiler arrangement can also serve to melt bottle contents on a stove top.
  • Pour liquified agar into each plate, anticipated to need for the test, and cover the bottom to about 6-7mm. Allow to harden or firm up at room temperature (24 hours or less). Plates are now ready to inoculate or refrigerate for later use.
  • To begin (the test), wipe swab over item to be tested.
  • Wipe the swab on the agar surface back and forth or in a pattern - lines, curves, figures - and label each dish with pattern description and a dish number.
  • Repeat steps 3 and 4 for each Petri dish in the test.
  • Cover the agar test dishes and secure lid with tape.
  • Store dishes upside down so condensation does not disturb bacteria growth. Keep in a warm, draft-free place as on top of a refrigerator.
  • Check and observe dishes after about two days. Any discoloration, especially along swab pattern, represents bacterial growth.